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In many instances, intraspecific discrimination (biotyping) of yeast and yeast-like microorganisms into specific subgroups (biotypes or genotypes) or the distinction of individual strains is needed, especially in epidemiological studies examining the dissemination of pathogens, or for effective protection of patented strains. For these needs many molecular methods can be employed such as: restriction fragment length polymorphism of mitochondrial DNA (mtDNA-RFLP), PFGE, randomly amplified polymorphic DNA (RAPD), PCR amplification with intron splice site primers or delta elements, simple sequence repeats (SSRs) and micro-satellite typing.

In addition, several non-molecular techniques can also be used for yeast biotyping, such as modified ELISA systems, determination of isoenzyme patterns, analysis of specific enzymatic activities, antibiogram typing, etc. Another easy and effective non-molecular method for yeast biotyping could be the determination of killer sensitivity patterns (KSPs) towards a panel of selected killer toxins.

One of the scopes of DBVPG Collection is the development of simple "fingerprinting" procedures (e.g. determination of DNA-banding pattern with microsatellites or specific probes, killer sensitivity patterns, etc.) capable of discriminating with certainty yeast and yeast-like strains within the species.

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