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Some media for yeast cultivation

Gorodkowa agar-modified
Glucose 0.1 %
Peptone 5.0 %
NaCl 0.5 %
Agar 2.0 %
Sterilize at 121°C for 15 minutes

ME3 (3% Malt extract agar)
Malt extract 3.0%
Agar 1.5%
Sterilize at 121°C for 15 minutes

ME5 (5% Malt extract agar)
Malt extract 5.0%
Agar 1.5%
Sterilize at 121°C for 15 minutes

MYA
Yeast extract 0.3%
Malt extract 0.3%
Peptone 0.5%
Glucose 1.0%
Agar 1.5%
Sterilize at 121°C for 15 minutes

Medium for the revitalization of lyophilized cultures
Yeast extract 0.5%
Peptone 0.5%
Glucose 4.0%
Sterilize at 121°C for 15 minutes

McClarey's acetate agar
Glucose 0.1 %
Potassium chloride 0.18%
Yeast extract 0.25%
Sodium acetate 0.82%
Agar 1.5%
Sterilize at 121°C for 15 minutes

PDA Potato extract 0.23 L
Glucose 20 g
Agar 15 g
Distilled, deionized water 0.77 L
pH = 6.6
Sterilize at 121°C for 15 minutes
Potato extract
Wash 5 kg of peeled and sliced potatoes and grind in a mincer.
Make a mixture using 300 mL of water with 100 g potatoes
After one night at 4 C, filter through a cloth
Adjust the liquid to pH 6.6
Sterilize 60 min at 120°C

Synthetic grape must
(Use for fermentation power trials)
Yeast Nitrogen Base (Difco) 13.7 g
Glucose 24 % (w/v)
pH = 3.0
Sterilize by Tyndalization (30 min at 100°C for three consecutive days)

YEG
Yeast extract 0.5 %
Glucose 5.0 %
Sterilize at 121°C for 15 minutes

YEPG
Yeast extract 1.0%
Peptone 1.0%
Glucose 2.0%
Sterilize at 121°C for 15 minutes

YEPG Agar
Yeast extract 1.0%
Peptone 1.0%
Glucose 2.0%
Agar 1.5%
Sterilize at 121°C for 15 minutes

YEPG + 15 % NaCl
Yeast extract 1.0 %
Peptone 1.0 %
Glucose 2.0 %
NaCl 15.0 %
Agar 1.5
Sterilize at 121°C for 15 minutes

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